Platelet aggregation in whole blood from patients with Glanzmann's thrombasthenia

Platelet aggregation in whole blood from patients with Glanzmann's thrombasthenia.

We examined platelet aggregation in platelet-rich plasma (PRP) and in whole blood from two patients with Glanzmann's thrombasthenia. In PRP, aggregation was measured by monitoring the changes in light absorbance that occurred in response to aggregating agents; to measure platelet aggregation in whole blood, we used a platelet counting technique. In PRP, the patients' platelets showed defective ...

Patients With Glanzmann Thrombasthenia Lacking Platelet Glycoprotein

Background—Platelets have been suggested to play a role in the early development of atherosclerosis. As one test of this hypothesis, we assessed whether patients with Glanzmann thrombasthenia who lack platelet glycoprotein IIb 3 (GPIIb/IIIa) complexes or both IIb 3 and the more ubiquitous v 3 cell membrane complexes are protected from development of atherosclerosis. Methods and Results—Seven pa...

Propofol inhibits in vitro platelet aggregation in human whole blood.

To help clarify the mechanism of propofol-induced vasodilation, we investigated whether propofol, at concentrations ranging from 10(-6) to 10(-3) M, inhibited platelet aggregation in human whole blood. Propofol inhibited platelet aggregation induced by adenosine diphosphate, collagen, or arachidonic acid in a concentration-dependent manner, with a 50% inhibited concentration (micromol/L) of 136...

Density of Platelet GPIIb-IIIa and Bleeding Severity in Iranian Patients with Glanzmann’s Thrombasthenia

Analysis of Human Platelet Glycoproteins lib-Illa and Glanzmann’s Thrombasthenia in Whole Blood by Flow Cytometry

Antibodies that bind to human platelet membrane glycoproteins llb and lIla were used to develop methods for analyzing platelet membrane components by flow cytometry. Platelets were tentatively identified by their lowintensity light scatter profiles in whole blood or plateletrich plasma preparations. Identification of this cell population as platelets was verified by using plateletspecific antib...